Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

1 – 15 of 2,872 results

PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™

Commonly used buffer

Pricing & Availability
Specifications

Content And Storage	Room temperature
Form	Liquid

Corning™ Cell Culture Buffers: Dulbecco's Phosphate-Buffered Salt Solution 1X

With low endotoxin content

Promotions are available

Pricing & Availability
Specifications

Dilution	1 X
Product Type	DPBS

Invitrogen™ NuPAGE™ MOPS SDS Running Buffer (20X)

Made with high-purity reagents and are strictly quality controlled.

Promotions are available

Pricing & Availability
Specifications

Formulation	50 mM MOPS, 50 mM Tris Base, 0.1% SDS, 1 mM EDTA, pH 7.7, 0.01-0.09% N,N-dimethylformamide
Physical Form	Liquid
pH	7.7
Chemical Name or Material	Running Buffer
Recommended Storage	Contents: NuPAGE™ MOPS SDS Running Buffer (20X) Storage: +4°C to 25°C
Concentration	20X
Product Line	NuPAGE

TBS, Tris Buffered Saline, 1X Solution, pH 7.4, Molecular Biology, Fisher BioReagents

Pricing & Availability
Specifications

Boiling Point	100°C
Name Note	1X Solution, pH 7.4
CAS	77-86-1
Protease	Protease free
Color	Colorless
Physical Form	Liquid
Chemical Name or Material	Tris Buffered Saline
Grade	Molecular Biology
Synonym	TBS
DNase	DNase free
Recommended Storage	RT
ChemAlert Storage Symbol	Gray

TBE Buffer, Tris-Borate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents™

CAS: 60-00-4,10043-35-3,7732-18-5,77-86-1 Synonym: TBE

Pricing & Availability
Specifications

CAS	60-00-4,10043-35-3,7732-18-5,77-86-1
Synonym	TBE

Corning™ Cell Culture Phosphate Buffered Saline (1X)

With low endotoxin content. Without calcium and magnesium.

Pricing & Availability
Specifications

Dilution	1 X
Content And Storage	2° to 30°C
Form	Liquid
Formulation	Potassium Dihydrogen Phosphate 0.144g/L, Sodium Chloride 9g/L, Disodium Phosphate 0.795g/L
pH	7.3 to 7.5

Gibco™ PBS (10X), pH 7.4

Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Content And Storage	Storage conditions: 15°C to 30°C Shipping conditions: Room temperature Shelf life: 24 months from date of manufacture
Form	Liquid
Color	White
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Green Features	Sustainable packaging
For Use With (Application)	Mammalian Cell Culture
Sterility	Sterile-filtered
Phenol Red Indicator	No Phenol Red
Osmolality	2800 - 3100 mOsm/kg
Dilution	10 X
Additive	No Sodium Pyruvate
Product Type	Phosphate Buffered Saline (PBS)
Classification	Animal Origin-Free
pH	7.4
Solution Type	Phosphate Buffered Saline
Product Line	Gibco™

Corning™ Molecular Biology Reagents

Used in molecular biology, nucleic acid purification applications

Pricing & Availability

TBS, Tris Buffered Saline, 10X Solution, pH 7.4, Molecular Biology, Fisher BioReagents™

Pricing & Availability
Specifications

Boiling Point	100°C
Color	Colorless
Physical Form	Liquid
Chemical Name or Material	Tris Buffered Saline
Grade	Molecular Biology
DNase	DNase free
ChemAlert Storage Symbol	Gray
Name Note	10X Solution
Concentration or Composition (by Analyte or Components)	10X solution contains 1.37M Sodium Chloride, 0.027M Potassium Chloride, and 0.25M Tris/Tris-HCl.
CAS	7732-18-5
Protease	Protease free
pH	7.5
Synonym	TBS
Purity Grade Notes	DNase-, RNase- and Protease-Free
Recommended Storage	RT

Gibco™ PBS, pH 7.4

PBS (phosphate buffered saline) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Form	Liquid
Color	Clear
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Green Features	Sustainable packaging
For Use With (Application)	Washing Cells Before Dissociation; Diluting Cells for Counting and Preparing Reagents; Transporting Cells or Tissue
Sterility	Sterile-filtered
Phenol Red Indicator	No Phenol Red
Osmolality	280 - 315 mOsm/kg
Dilution	1 X
Additive	No Sodium Pyruvate
Product Type	Phosphate Buffered Saline (PBS)
Classification	Animal Origin-Free
pH	7.4
Solution Type	Phosphate Buffered Saline
Product Line	Gibco™

Gibco™ DPBS, no calcium, no magnesium

Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.

Pricing & Availability
Specifications

Form	Liquid
Product Type	DPBS
Classification	Animal Origin-Free
pH	7.0 to 7.3
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
For Use With (Application)	Mammalian Cell Culture
Sterility	Sterile-filtered
Phenol Red Indicator	No Phenol Red
Osmolality	270 - 300 mOsm/kg
Solution Type	Dulbecco's Phosphate Buffered Saline

Invitrogen™ PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free

PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Dilution	10 X
Content And Storage	Store at room temperature.
Form	Liquid
Product Type	Phosphate Buffered Saline (PBS)
pH	7.4
Product Line	Ambion™

Gibco™ CTS™ DPBS, without calcium chloride, without magnesium chloride

Gibco Cell Therapy Systems (CTS) DPBS is the classic Dulbecco's phosphate-buffered saline. This particular formulation lacks calcium, magnesium, and phenol red.

Promotions are available

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Dilution	1X
Content And Storage	Store at 15–30°C.
Additive	No Calcium, No Magnesium
Form	Liquid
Product Type	DPBS
Classification	AOF
Manufacturing Quality	ISO13485, MDSAP, FDA-registered, 21 CFR820
Green Features	Sustainable packaging
Shelf Life	36 months from date of manufacture
For Use With (Application)	Cell and Gene Therapy Research, Development, and Manufacturing
Sterility	Sterile-filtered
Solution Type	Dulbecco's Phosphate Buffered Saline

Tris Hydrochloride, 1M Solution (pH 7.0/Mol. Biol.), Fisher BioReagents™

CAS: 77-86-1,7732-18-5,1185-53-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tris-HCl PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1,7732-18-5,1185-53-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
SMILES	C(C(CO)(CO)N)O
Synonym	Tris-HCl
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

Thermo Scientific Chemicals Potassium phosphate, 0.5M buffer soln., pH 7.4

CAS: 7778-77-0 Molecular Formula: H2KO4P Molecular Weight (g/mol): 136.08 MDL Number: MFCD00011401 MFCD00147253 InChI Key: GNSKLFRGEWLPPA-UHFFFAOYSA-M IUPAC Name: potassium dihydrogen phosphate SMILES: [K+].OP(O)([O-])=O

Pricing & Availability
Specifications

CAS	7778-77-0
Molecular Weight (g/mol)	136.08
MDL Number	MFCD00011401 MFCD00147253
SMILES	[K+].OP(O)([O-])=O
IUPAC Name	potassium dihydrogen phosphate
InChI Key	GNSKLFRGEWLPPA-UHFFFAOYSA-M
Molecular Formula	H2KO4P

Life Science Buffers

Life Science Buffers

Filtered Search Results

Narrow Results

Corning™ Cell Culture Phosphate Buffered Saline (1X) Supplier Diversity PartnerSmall and/or diverse supplier based on Federal laws and SBA requirements.Learn More

Gibco™ PBS (10X), pH 7.4 Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Corning™ Molecular Biology Reagents SureTRACESupports traceability with guaranteed access to certificates and proactive change notifications.Learn More Supplier Diversity PartnerSmall and/or diverse supplier based on Federal laws and SBA requirements.Learn More

Gibco™ DPBS, no calcium, no magnesium Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Corning™ Cell Culture Phosphate Buffered Saline (1X)

Gibco™ PBS (10X), pH 7.4

Corning™ Molecular Biology Reagents

Gibco™ DPBS, no calcium, no magnesium