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BD CompBead Anti-Mouse Ig, κ/Negative Control Particles Set
Used to optimize fluorescence compensation settings for multicolor flow cytometric analyses. BD™ Anti-Mouse Ig, κ/Negative Control Particles Set includes two sets of polystyrene microparticles to distinctly stain positive and negative populations, especially when using tandem dye conjugates.
Supplier: BD 552843
Description
The set provides two populations of microparticles:
- BD™ CompBeads Anti-Mouse Ig, κ—Binds any mouse κ light chain-bearing immunoglobulin
- BD™ CompBeads Negative Control—Has no binding capacity
When mixed together with a fluorochrome-conjugated mouse antibody, the CompBeads provide distinct positive and negative (background fluorescence) stained populations which can be used to set compensation levels manually or using instrument set-up software.
Since the compensation adjustments are made using the same fluorochrome-labeled antibody to be used in the experiment, this method allows the investigator to more accurately establish compensation corrections for spectral overlap for any combination of fluorochrome-labeled antibodies (without having to use valuable tissue samples or hard-dyed beads with potentially mismatched fluorescence spectra).
Use of the BD™ CompBeads is highly recommended for use in all experiments using tandem dye (i.e., PE-Cy™7, APC-Cy™7, etc.) conjugates, which may have distinct spectral characteristics for each conjugate.
Specifications
| Mouse Ig, κ | |
| Unconjugated | |
| Unpurified | |
| Primary | |
| Liquid |
| Flow Cytometry | |
| CompBead Anti-Mouse Ig, kappa Particles CompBead Negative Control (FBS) Compensation Particles |
|
| 6 mL Each | |
| Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
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